Roadsides in North and Central Florida harbor a large number of milkweed populations important to the monarch butterfly. A total of 303 roadway locations had one or more plants of the target species. A comprehensive geo-referenced Excel spreadsheet of all populations locations and related population data was developed and submitted to the Florida Department of Transportation. Communication/dissemination and vegetation management recommendations are presented to help maximize the availability and productivity of roadside milkweed populations for monarch and pollinator conservation.