This paper describes autonomous detection of milkweed plants using cameras mounted to vehicles. The detections were used to calculate approximate densities of milkweed plants in geo-referenced locations based on global positioning system point correspondences of recorded images. Probability-of-count distributions are compared for the actual milkweed plant locations near roadsides. This is one of the first examples of using automated milkweed plant detection and density mapping using a vehicle-mounted camera.